GSM4629052: Kuramochi CTCF 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Gonad
Cell type
KURAMOCHI
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Ovarian cancer cell line
cell type
Ovarian cancer cell line
cell line
Kuramochi
chip antibody
CTCF
antibody catalog
61311
antibody lot
N/A
young_id
N/A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
CTCF ChIP-Seq was performed as previously described (Schmidt et al., Methods, 2009) using 25 μg chromatin and 5 μg anti-CTCF antibody (Active Motif, catalogue number 61311) and chromatin sheared to 100-300 bp fragments using a Covaris E220 evolution Focused Ultrasonicator. ChIP libraries were constructed using the Kapa Hyper Library Preparation kit, quantified and sequenced on an Illumina NextSeq 500 sequencer. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.